Coding
Part:BBa_K1641000
Designed by: Pai Li Group: iGEM15_SYSU_CHINA (2015-09-08)
Recombinase Vcre, E. coli-and-yeast coding optimized
This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence VloxP, which do not cross-interfering with Cre-LoxP system. Vcre has the invertase activity that invert the the sequence between two anti-parallel Vloxp. The original Vcre is discovered through genome screening of Vibrio sp. 0908, yet this coding sequence is redesigned into E. coli-and-yeast coding preference from original protein sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |